Team, Visitors, External Collaborators
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New Software and Platforms
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Section: New Results

Reconstruction of macroscopic forms from images and characterization of their variability

Participants : Ayan Chaudhury, Christophe Godin, Jonathan Legrand, Katia Mirande.

To study the variability of macroscopic forms resulting from development, it is necessary to both develop digital reconstruction methods, typically based on image acquisitions, and statistical tools to define notions of distance or average between these forms. The automatic inference of computational representations of forms or organ traits from images of different types is therefore an essential step, for which the use of prior knowledge can be very beneficial. Realistic synthetic models of forms can guide the reconstruction algorithms and/or assess their performances. Computational representations of forms can then be used to analyze how forms vary at the scale of a population, of a species or between species, with potential applications in species identification and genetic or environmental robustness estimation.

Automatized characterization of 3D plant architecture. The digital reconstruction of branching and organ forms and the quantification of phenotypic traits (lengths of internodes, angles between organs, leaf shapes) is of great interest for the analysis of plant morphology at population scale. In collaboration with the ROMI partners from Sony CSL, Paris, we develop an automated processing pipeline that involves the 3D reconstruction of plant architecture from RGB image acquisitions performed by a robot, and the segmentation of the reconstructed plant into organs. We aim at releasing both hardware schematics and the developed software for image reconstruction to be used as cheap open-source solution to phenotype plants. In addition, to provide validation data for the pipeline, we designed a generative model of Arabidopsis thaliana simulating the development of the plant architecture at organ scale. This model was used to develop the method for the measurement of angles of organs and test its accuracy:

In both cases, knowing the generated phenotypic traits or the model shape allow to test the pipeline ability to reconstruct the plant and quantify its traits of interest

The developed reconstruction and quantification pipeline is not made from scratch but aggregate a number of available third party libraries and codes in addition to three active research topics: spectral clustering, skeleton extraction, and ML segmentation. In a second phase, the model will be used to generate training data for machine learning techniques introduced in the reconstruction methods. This work is part of the ROMI project.