Section: Software

Keywords : creation and update database (update database, creation database), transcriptomic technique.

RFDD

Participant : Guy Perrière [ correspondent ] .

In collaboration with Helene Simonnet (Centre de Genetique Moleculaire et Cellulaire, UMR CNRS 5534, Lyon), we have developed a web service for bioinformatic analysis of RFDD (Restriction Fragment Differential Display) results. RFDD is a transcriptomic technique derived from AFLP (Analysis of Fragment Length Polymorphism). This service uses a relational database of restriction fragments obtained by in silico digestion of all human and rat transcribed sequences present in the RefSeq database. We have developed software for the creation and update of this database and for providing web access to it (http://pbil.univ-lyon1.fr/software/RFDD/ ). A first biological result has been derived from this tool by analysis of transcriptome data from rat under hypoxic conditions [24] .